5μm 120Å C18-Alkaline HPLC Column from manufacturer

Column: XChromaTM HPLC Column, C18-Alkaline, 4.6 ×250 mm, 5 µm
Mobile Phase:
A:0.5 mol/L KH2PO3(adjust pH to 3.5 with H3PO4):MeOH:water=10:30:60
B:0.5 mol/L KH2PO3(adjust pH to 3.5 with H3PO4):MeOH:water=10:50:40
Flow Rate: 1.0 ml/min
Detector: 225nm
Temperature: 30℃
Injection Volume: 20μl